Switched-battery boost-multilevel inverter with GA optimized SHEPWM for standalone application

This paper presents a boost-multilevel inverter design with integrated battery energy storage system for standalone application. The inverter consists of modular switched-battery cells and a full-bridge. It is multifunctional and has two modes of operation: the charging mode which charges the battery bank and the inverter mode which supplies AC power to the load. This inverter topology requires significantly less power switches compared to conventional topology such as cascaded H-bridge multilevel inverter, leading to reduced size/cost and improved reliability. To selectively eliminate low-order harmonics and control the desired fundamental component, nonlinear system equations are represented in fitness function through the manipulation of modulation index and the Genetic Algorithm is employed to find the optimum switching angles. A 7-level inverter prototype is implemented and experimental results are provided to verify the feasibility of the proposed inverter design

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Optimal VF-PDPC of grid connected inverter under unbalanced and distorted grid voltages

In this article, an optimal virtual flux predictive direct power control (VF-PDPC) is proposed for three-phase grid connected voltage source inverter (VSI) operating under unbalanced and distorted grid supply voltage. The concept of virtual flux and extension pq theory is introduced in the conventional PDPC, where the former enhances its resilience toward distorted supply voltage while the latter enhances its resilience toward unbalanced supply voltage. The inherent advantages of the conventional PDPC scheme, such as simple implementation, fast dynamic responses, and no demand of modulators are retained. Getting rid of the complicated positive and negative sequence extraction of grid components, power compensation, or any tuning work, the proposed optimal VF-PDPC achieves excellent power tracking capability, and sinusoidal grid currents with low harmonics under various grid voltage conditions. Simulation results are presented to verify the effectiveness of the proposed control scheme when compared to the conventional PDPC, conventional VF-PDPC, and optimal PDPC

Improved single-phase split-source inverter with hybrid quasi-sinusoidal and constant PWM

A single stage topology of three phase boost inverter known as split-source inverter (SSI) has recently been introduced in literature. This topology suffers from high frequency current commutations across two diodes and complicated analysis since the inductor is charged with variable duty cycle. This paper presents a single phase version of SSI with improvements in inverter topology as well as the PWM technique. An inductor is connected to two MOSFETs operating at fundamental frequency to boost the voltage from input source to dc link voltage. In the proposed hybrid quasi-sinusoidal and constant PWM, one of the full-bridge legs undergoes constant duty cycle switching while the other one undergoes sinusoidally varying duty cycle switching, with the former is accountable for charging and discharging of inductor while the latter is accountable for producing ac output. Therefore, the proposed topology with hybrid quasi-sinusoidal and constant PWM exhibit the merit of simplicity since the control of dc link voltage and ac output are detached within the single stage topology. It is not liable to the undesired high frequency current commutation. In addition, a wide range of ac output voltage is achievable in either buck or boost operation. Theoretical analysis are presented and verified through simulation and experimental results

A symmetrical cascaded compact-module multilevel inverter (CCM-MLI) with pulsewidth modulation

Cascaded H-bridge (CHB) multilevel inverters (MLIs) have been widely used for power electronics systems. While high-voltage blocking across power switches is not a constraint for low voltage applications, the research trend has been oriented to the design of more compact module topologies as an alternative for CHB. Despite the generation of more voltage levels with reduced switch count, the existing module topologies in recent literature take no account of the freewheeling current path during dead-time, thus, inducing multistep jumps in voltage levels and giving rise to undesirable voltage spikes. Addressing this concern, this paper proposes two symmetrical compact-module topologies for cascaded MLI, where freewheeling current path during dead-time is provided for smooth transition between voltage levels to prevent voltage spikes. The proposed 7-level and 13-level compact-modules demonstrated low number of conducting switches for all voltage levels. Comprehensive analysis and comparison with the latest module topologies are conducted. To validate the operation of the proposed compact-module topologies, simulation and experimental results are presented

Hybrid cascaded multilevel inverter (HCMLI) with improved symmetrical 4-level submodule

This letter proposes an improved symmetrical 4-level submodule as a basic cell for generating multiple dc voltage levels. A hybrid cascaded multilevel inverter (HCMLI) topology is formed by the combination of n submodules and a full-bridge. A comparative analysis against the recent multilevel inverters reveals that the proposed topology requires less number of switches and dc sources. In addition, the proposed submodule reduces the number of conducting switch and gate driver requirements compared to the widely used half-bridge submodule. To validate the operation of the proposed HCMLI topology, experimental results of a 9-level single-phase inverter controlled by selective harmonic elimination pulse-width-modulation is presented

Cytotoxicity and transformation of C3H10T1/2 cells induced by areca nut components

Betel quid (BQ) chewing is popular in Taiwan and many other countries. There are about 200–600 million BQ chewers in the world. BQ chewing is one major risk factor of oral cancer and oral submucous fibrosis (OSF). While areca nut (AN), a main component of BQ, exhibits genotoxicity, its transformation capacity and its role in the initiation and promotion stages of carcinogenesis are not fully clear. Methods: Mouse C3H10T1/2 cells were exposed to AN extract (ANE) for 24 hours. Cytotoxicity was evaluated by colony forming efficiency. For the transformation assay, C3H10T1/2 cells were exposed to ANE for 24 hours and then incubated in medium with/without 12-O-tetradecanolylphorbol-13-acetate (TPA; a tumor promoter) for 42 days. Cells were stained with Giemsa and type II and type III transformed foci were counted for analysis of the transformation capacity of ANE. Results: ANE exhibited cytotoxicity to C3H10T/12 cells at concentrations higher than 320 μg/mL as shown by a decrease in colony numbers. ANE (80–640 μg/mL) alone mildly stimulated the transformed foci formation (p > 0.05). In the presence of TPA, ANE (80–640 μg/mL) markedly stimulated the transformed foci formation. The percentage of dishes with foci increased from 0% in controls to 20% in ANE (80 μg/mL and 320 μg/mL)-treated groups and further increased to 65–94% in ANE plus TPA groups. Conclusion: These results indicate that ANE is a weak complete carcinogen. ANE is an effective tumor initiator and can induce malignant transformation of C3H10T1/2 cells in the presence of a tumor promoter. ANE may be involved in multistep chemical carcinogenesis by its malignant transformation capacity

Functional Connectivity Density Mapping of Depressive Symptoms and Loneliness in Non-Demented Elderly Male

Background: Depression and loneliness are prevalent and highly correlated phenomena among the elderly and influence both physical and mental health. Brain functional connectivity changes associated with depressive symptoms and loneliness are not fully understood. Methods: A cross-sectional functional MRI study was conducted among 85 non-demented male elders. Geriatric depression scale-short form (GDS) and loneliness scale were used to evaluate the severity of depressive symptoms and loneliness, respectively. Whole brain voxel-wise resting-state functional connectivity density (FCD) mapping was performed to delineate short-range FCD (SFCD) and long-range FCD (LFCD). Regional correlations between depressive symptoms or loneliness and SFCD or LFCD were examined using general linear model (GLM), with age incorporated as a covariate and depressive symptoms and loneliness as predictors. Results: Positive correlations between depressive symptoms and LFCD were observed in left rectal gyrus, left superior frontal gyrus, right supraorbital gyrus, and left inferior temporal gyrus. Positive correlations between depressive symptoms and SFCD were observed in left middle frontal gyrus, left superior frontal gyrus, bilateral superior medial frontal gyrus, left inferior temporal gyrus, and left middle occipital region. Positive correlations between SFCD and loneliness were centered over bilateral lingual gyrus. Conclusion: Depressive symptoms are associated with FCD changes over frontal and temporal regions, which may involve the cognitive control, affective regulation, and default mode networks. Loneliness is associated with FCD changes in bilateral lingual gyri that are known to be important in social cognition. Depressive symptoms and loneliness may be associated with different brain regions in non-demented elderly male

Effect of Bcl-2 rs956572 polymorphism on age-related gray matter volume changes.

The anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl-2) gene is a major regulator of neural plasticity and cellular resilience. Recently, the Bcl-2 rs956572 single nucleotide polymorphism was proposed to be a functional allelic variant that modulates cellular vulnerability to apoptosis. Our cross-sectional study investigated the genetic effect of this Bcl-2 polymorphism on age-related decreases in gray matter (GM) volume across the adult lifespan. Our sample comprised 330 healthy volunteers (191 male, 139 female) with a mean age of 56.2±22.0 years (range: 21-92). Magnetic resonance imaging and genotyping of the Bcl-2 rs956572 were performed for each participant. The differences in regional GM volumes between G homozygotes and A-allele carriers were tested using optimized voxel-based morphometry. The association between the Bcl-2 rs956572 polymorphism and age was a predictor of regional GM volumes in the right cerebellum, bilateral lingual gyrus, right middle temporal gyrus, and right parahippocampal gyrus. We found that the volume of these five regions decreased with increasing age (all P<.001). Moreover, the downward slope was steeper among the Bcl-2 rs956572 A-allele carriers than in the G-homozygous participants. Our data provide convergent evidence for the genetic effect of the Bcl-2 functional allelic variant in brain aging. The rs956572 G-allele, which is associated with significantly higher Bcl-2 protein expression and diminished cellular sensitivity to stress-induced apoptosis, conferred a protective effect against age-related changes in brain GM volume, particularly in the cerebellum